Journal: STAR Protocols
Article Title: Generation of embryo-like structures from mouse embryonic stem cells treated with a chemical inhibitor of SUMOylation and cultured in microdroplets
doi: 10.1016/j.xpro.2023.102573
Figure Lengend Snippet:
Article Snippet: Fill two 1 mL glass syringes (SGE), equipped with PTFE tubing (0.56 mm ID and 1.07 mm OD, Adtech), with a FC-40/0.5% RAN solution. c. Equip a low-pressure cross body (Idex, 1/4–28 flat-bottom ports) with Idex Flangeless Short Head Fitting Assemblies (1/4–28 flat-bottom connection for 1/16″ OD Tubing) and with PFA resin tubing (Idex 1507L) with an inner diameter of 0.040″ and outer diameter of 1/16″, connected with three silicone tubing segments (ID 1.0 mm, Thermo Fisher Scientific).
Techniques: Recombinant, Knock-Out, Membrane, Software, Chromatography, Polymer, Sterility